INDEL-Typing of <i>Yersinia pseudotuberculosis</i> Strains

نویسندگان

چکیده

The aim of this study was to develop a new method intraspecific genetic differentiation Yersinia pseudotuberculosis, based on the detection INDEL-markers using PCR. Materials and methods. Analyzed were 308 strains from NCBI database 15 sequenced within frames study. nucleotide sequences determined MiSeq technology platform. genomes in work, as well database, assessed silico PCR with 7 pairs primers designed As result comparison genome-wide 22 Y. pseudotuberculosis author’s software (GenExpert), selected that make it possible effectively distinguish between causative agent pseudotuberculosis. Based these markers, synthesized for analysis different Analysis 323 70 vitro allowed dividing them into 30 groups. Comparison results sili co confirmed possibility proposed . data obtained, dendrogram reflecting phylogenetic relations constructed. When analyzing distribution by various clusters groups, number patterns revealed. Conducted show INDEL-typing can be used

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ژورنال

عنوان ژورنال: Problemy osobo opasnyh infekcij

سال: 2023

ISSN: ['0370-1069', '2658-719X']

DOI: https://doi.org/10.21055/0370-1069-2022-4-102-109